MADAN KUMAR PAUDEL, PhD

Bio- medical Scientist

Contact

PHONE:

977-9860230003

EMAIL:

[email protected]

SKILLS

Monoclonal Antibodies

Immunochromatographic Assay

Rapid Diagnostic Test

AMR / Cell Culture

MALDI-TOF-MS

TLC/GLC/HPLC

ESI/MS& GC/MS

H-NMR and C-NMR

Protein GF column

All Chromatography

ELISA, Enzyme Assays

PCR / qPCR & sequencings’

Genetic engineering

Mice handling & inoculations

Practical Laboratory Skills

Data Management SPSS

LANGUAGE PROFICIENCY:

English

Nepali

Japanese

Hindi

(Good ability to speak & write)

GRANTS, FUNDS & SCHOLARSHIPS:

NAST Brain Pooling fund, Nepal (2020/2021)

University Grant Commission (UGC) fund, Nepal (2020/2021)

Kyushu Young Scientific Research Fund, Japan (2015/4—2017/3)

Kobayashi International Scholarship Foundation, Japan (2012/4—2015/3)

Sasakawa Scientific Research Fund, Japan (2012/4—2013/3)

Rotary Yoneyama Kinen Scholarship, Japan (2010/4—2012/3)

Kyushu University Student Funds, Japan (2011/10-2012/3)

JASSO Scholarship, Japan (2008/4—2010/3)

STRENGTH:

Strong belief in professionalism, positive attitude and dedication, willingness to learn and implement.

Quick adoptability to any environment.

Flexible and Responsible.

Capability of understanding and implementing modern techniques quickly.

Self-Motivating and hardworking.

Optimistic and Sincere.

EXTERNAL, SUPERVISIONS, EVALUATIONS & PEER REVIEW

Paper peer reviewer for Ethnobotany Journal

Paper, peer reviewer for Economy Botany Journal

As External 2 for Master at CDC, TU, Kritipur

PhD Students 1 as co-supervisor at CDC, TU, Kritipur

Master Students 3 as co-supervisors at CDC, TU, Kritipur

Master Thesis Evaluation more than dozens in NAST, Lalitpur

Research Grant Evaluation more than dozens in NAST, Lalitpur

PhD Thesis Grant Evaluation more than dozens in NAST, Lalitpur

Research Grant Evaluation for University Grant Commission (UGC), Bhaktapur.

EDUCATION

Kyushu University, Japan

PhD in Pharmaceutical Sciences in 2017.

WORK EXPERIENCE

Nepal Health Research Council (NHRC), Consultant, till date

Since 21 August, 2022 working as Consultant for Review of Ethical guidelines for the care and use of animals in health research in Nepal along with preparation of standard operating procedure (SOP) for implementation of guidelines, workshops, technical working group (TWG) meetings, seminars and trainings.

Nepal Academy of Science & Technology (NAST), Senior Scientist

Since 14 March 2021 to 15 March 2022 as a Senior Scientist followed with the evaluation and monitoring of different climate and environmental changes in herbal medicinal plants for monoclonal antibody against Gurjo.

Phytomax Care International Pvt. Ltd., Director, R&D

Since 1 July 2017 to 15 March 2022 as a Director, R&D followed with the evaluation and monitoring of different herbal cosmetic products using medicinal plants.

Tribhuwan University, Centre Department of Chemistry, Kirtipur, Kathmandu

Co-PI of UGC on research no Cov76/77-02. Works on AMR and Covid- l9: Investigation on Secondary Metabolites from Natural Products through Computational Chemistry and immunochemistry approach.

Karnali College, Kathmandu, Nepal 

Since August 2017 to till as faculty Lecturer in Purbanchal University.

M.S. Technique, Japan 

Since April 2012 to June 2017 as Senior Business Researcher in Pharmacy with the responsibility of monitoring and evaluation of cosmetic products.

PUBLICATIONS: 14

<Original Paper>

GyawaliR, Paudel P, Basyal D, Setzer W, Lamichhane S, Paudel M K, Gyawali S, Khanal Prajwal. A Review on Ayurvedic Medicinal Herbs as Remedial prospective for COVID-19. JKAHS 2020;3

Paudel M.K., Sakamoto S., Morimoto S., Tanaka H., An overview and comparison of a recombinant antigen-binding fragment and an antigen-binding fragment from a monoclonal antibody against wogonin glucuronide, Journal of Natural Medicines, 71, 4, 703-710, 2017.09.

Paudel M.K., Sakamoto S., Shirota O., Morimoto S., Tanaka H., An immunochromatographic assay for rapid detection of salvinorin A, Journal of Immunoassay and Immunochemistry, 38, 4, 438-448, 2017.06.

Paudel M.K., Sakamoto S., Huy L.V., Tanaka H., Miyamoto T., Morimoto S., The effect of varying the peptide linker length in a single chain variable fragment antibody against wogonin glucuronide, Journal of Biotechnology, 251, 47-52, 2017.06.

Paudel M.K., Sakamoto S., Huy L.V., Tanaka H., Miyamoto T., Akihito T., Morimoto S., Development of an immunoassay using an anti-wogonin glucuronide monoclonal antibody, Journal of Immunoassay and Immunochemistry, 38, 5, 457-470, 2017.10.

Tanaka H., Paudel M.K., Shirota O., Morimoto S., Enzyme Immunoassay for Salvinorin A (a Main Component in Salvia divinorum) Chapter in book: Neuropathology of Drug Addictions and Substance, pp.749-756, Apr 2016.

Sakamoto S, Yusakul G, Pongkitwitoon B, Paudel M.K., Tanaka H, Morimoto S. Simultaneous determination of soy isoflavone glycosides, daidzin and genistin by monoclonal antibody-based highly sensitive indirect competitive enzyme-linked immunosorbent assay. FOOD CHEMISTRY. 2015.

Paudel M.K., Shirota O, Sasaki-Tabata K, Tanaka H, Sekita S, Morimoto S. Development of an enzyme immunoassay using a monoclonal antibody against the psychoactive diterpenoid salvinorin A. JOURNAL OF NATURAL PRODUCTS 2013

Tanaka H, Paudel, M.K., Takei A., Sakoda J., Juengwatanatakul T., Sasaki-Tabata K., Morimoto S., Immunochemical Analysis of the Antimalarial Drugs Artemisinin and Artesunate, Article in Antibodies, Nov 2012.

Chao Z, Tan M, Paudel M.K., Sakamoto S, Ma L, Sasaki-Tabata K, Tanaka H, Shoyama Y, Xuan L, Morimoto S. Development of an indirect competitive enzyme-linked immunosorbent assay (icELISA) using highly specific monoclonal antibody against paclitaxel. JOURNAL OF NATURAL MEDICINE. 2012.

Tanaka H, Paudel, M.K., Takei A., Sakoda J., Juengwatanatakul T., Sasaki-Tabata K., Morimoto S.,Preparation of a reconbinant fab against the anti-malarial drugs, artemisinin and artesunate and their application in an ELISA,

Article in Planta Medica, Jul 2012

Paudel M.K., Shirota O, Sasaki-Tabata K, Tanaka H, Sekita S, Morimoto S., Development of an immunochemical differentiation method for Salvia divinorum, Article in Planta Medica, Jul 2012

Paudel, M.K., Takei A., Sakoda J., Juengwatanatakul T., Sasaki-Tabata K., Tanaka H, Morimoto S, Preparation of a single-chain variable fragment and a　recombinant antigen-binding fragment against anti-malarial drugs, artesunate and artemisinin,and the application for ELISA.　ANALYTICAL CHEMISTRY 2012.

Paudel M.K., Putalun W, Sritularak B, Morinaga O, Shoyama Y, Tanaka H, Morimoto S, Development of a combined technique using a one-step immunochromatographic assay and indirect competitive ELISA for the rapid detection of baicalin, ANALYTICA CHIMICA ACTA 2011.